Composite

Part:BBa_K592016:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-21)


B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 634
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 1641
    Illegal AgeI site found at 276
    Illegal AgeI site found at 1422
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1311
    Illegal BsaI.rc site found at 175


Design Notes

This constructs Strong RBS to ensure good expression.


Source

The components that made up of YF1 fusion protein comes from Bradyrhizobium japonicum and Bacillus subtilis. FixJ comes from the genome of Bradyrhizobium japonicum.

References